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넳 넲

黄俊

教授

校网主页: https://jszy.zust.edu.cn/swyhxgcxy/hj2/main.htm
邮箱：huangjun@zust.edu.cn

个人简介: 黄俊，政协第十三届浙江省委员会委员，二级教授、博士生导师，生物与化学工程学院院长。入选浙江省“万人计划”科技创新领军人才、浙江省高校领军人才（高层次拔尖人才）、浙江省151人才第二层次、浙江省高校优秀青年教师资助计划、浙江科技学院首批青年英才计划。兼任浙江省农产品化学与生物加工技术重点实验室副主任，浙江省食品学会常务理事、浙江省微生物学会理事、浙江省生物制药专业青年分委会副主任委员。获得2020年浙江省抗击新冠肺炎疫情先进个人、浙江省第五届师德先进个人、浙江省科技帮扶促调先进个人、浙江省优秀硕士论文指导教师、浙江省优秀毕业研究生、浙江大学优秀毕业研究生等。曾前往美国明尼苏达大学双城校区开展为期15个月的访问学者工作。主要学术兼职：国家自然科学基金通讯评审专家，浙江省、广东省、江苏省、安徽省、湖北省、河北省、重庆市自然科学基金以及科技计划项目通讯评审和会评专家。主持国家自然科学基金5项、浙江省重点研发领雁计划1项、浙江省重大科技专项1项、宁波市“科技创新2025”重大专项1项、国家重点研发计划子课题1项以及企业横向多项，研究方向涉及蛋白质分子设计和定向进化、检测仪器及配套试剂、合成生物学等领域。在国际学术期刊《Biotechnol Bioeng》《Biochemistry》等杂志发表学术论文80余篇，获中国轻工业联合会科学技术进步奖一等奖1项、浙江省自然科学三等奖1项、国家市场监管科研成果奖一等奖1项、中国石油和化学工业科学技术奖三等奖1项。

研究方向: 蛋白质分子设计和定向进化、检测仪器及配套试剂、合成生物学等领域。

开设课程: 生物学基础、生物催化与转化、生物工程导论、生物工程进展

科研项目：

纵向项目

[1] 国家自然科学基金（主持）：谷氨酸脱羧酶家族"分子开关"调控脱羧-氧化双功能性的分子机制研究（No. 32371316）

[2] 国家自然科学基金（主持）：基于蛋白质共进化功能区结构层次的转氨酶手性识别分子机制及构效关系研究（No. 32071268）

[3]国家自然科学基金（主持）：PLP依赖的II-氨基酸脱羧酶家族祖先基因序列重构及其趋异进化机制研究（No.31470793）

[4] 国家自然科学基金（主持）：乳酸菌谷氨酸脱羧酶的催化机理及其结构与功能关系研究（No.31240054）

[5]国家自然科学基金（主持）：乳酸菌中谷氨酸脱羧酶应答酸性pH环境胁迫的分子调控机制研究（No.30970638）

[6] 国家重点研发子课题（主持）：蛋白类生物产制品质量检测和计量溯源技术（No.2021YFF0600800）

[7] 浙江省重点研发计划（主持）：畜禽、水产投入品危害因子新型检测技术研究（No.2022C02049）

[8]浙江省重大科技专项（主持）：新型β-壳多糖及其衍生物系列制备关键技术研究及中试（No. 2008C14067）

[9] 浙江省重点研发计划子课题（主持）：农产品重要危害物快速准确检测关键技术研究与应用-浙江特色农产品重要危害物多目标快速准确检测关键技术研究和应用（No. 2020C02024）

[10] 宁波市“科技创新2025”重大专项（主持）：心血管疾病诊断用关键酶与抗体制备和试剂盒研发

[11] 浙江省“三农九方”农业科技协作计划揭榜挂帅项目（主持）：水产养殖中致病微生物全自动一体化核酸检测设备及配套试剂研发及产业化（No. 2023SNJF066）

[12] 杭州市新型冠状病毒感染的肺炎科研攻关项目（主持）：全自动移液耦合高通量核酸提取工作站关键技术研发及产业化

[13] 中国博士后科学基金（主持）：ω-转氨酶的分子改造及应用于三氟甲基胺的不对称合成（No.2015M581621）.

[14]浙江省“尖兵”研发攻关计划项目（参与）：生鲜农产品冷链物流智能化关键技术研究与应用（No. 2023C02006）

[15] 浙江省“领雁”研发攻关计划项目（参与）：跨境电商进口营养功能食品安全性评估和检测技术研究与应用（No. 2023C02037）

发表论文统计：

  1. Jiaren Cao, Fangfang Fan, Changjiang Lyu, Sheng Hu, Weirui Zhao, Jiaqi Mei, Shuai Qiu, Lehe Mei, Jun Huang. Pocket modification of ω-amine transaminase AtATA for overcoming the trade-off between activity and stability toward 1-acetonaphthone. Engineering, 2023, 30: 203-214. (IF 12.8)
  2. Yawen Rong,  Md Mehedi Hassan, Jizhong Wu, Shuo Chen , Wancheng Yang, Yunhao Li ,Jiaji Zhu, Jun Huang, Quansheng Chen. Enhanced detection of acrylamide using a versatile solid-state upconversion sensor through spectral and visual analysis. Journal of Hazardous Materials, 2024, 466 133369. (IF 13.6)
  3. Fangmei Liu, Kai Xia, Yuqing Chen, Ling Zhu, Lingzhi Zhu, Xuequn Zhao, Ruyi Sha, Jun Huang. Inhibition of hyphal formation together with biochar addition promotes erythritol production by Yarrowia lipolytica. Biotechnology and Bioengineering, 2024, DOI: 10.1002/bit.28704.
  4. Kai Xia, Yuqing Chen, Fangmei Liu, Xuequn Zhao, Ruyi Sha, Jun Huang. Adaptive responses of erythritol‑producing Yarrowia lipolytica to thermal stress after evolution. Applied Microbiology and Biotechnology, 2024, 108: 203.
  5. Qian Zhao, Jie Wang, Qing Li, Jiarong Zhang, Ruijun Hou, Zhihui Wang, Qian Zhu, Yifeng Zhou, Yue Chen, Jun Huang. Integrated transcriptome and metabolome analysis provide insights into the mechanism of saponin biosynthesis and its role in alleviating cadmium-induced oxidative damage in Ophiopogon japonicum. Plant Physiology and Biochemistry, 2024, 210: 108634.
  6. Shuai Qiu, Yu-Tong Cui, Tong-Tong Wang, Fang-Fang Fan, Chang-Jiang Lyu, Jun Huang. Stereoselective synthesis of (R)-(+)-1-(1-naphthyl)ethylamine by ω-amine transaminase immobilized on amino modified multi-walled carbon nanotubes and biocatalyst recycling. Enzyme and Microbial Technology, 2024, 174: 110378.
  7. Linyu Chen, Fangfang Fan, Meiyuan Yang, Linquan Wang, Yushuo Bai, Shuai Qiu, Changjiang Lyu, Jun Huang.Atomistic insight into the binding mode and self-regulation mechanism of IsPETase towards PET substrates with different polymerization degrees. Physical Chemistry Chemical Physics, 2023, 25: 18332-18345.
  8. Chun-Ning Wang, Shuai Qiu, Fang-Fang Fan, Chang-Jiang Lyu, Sheng Hu, Wei-Rui Zhao, Jia-Qi Mei, Le-He Mei, Jun Huang. Enhancing the organic solvent resistance of ω-amine transaminase for enantioselective synthesis of (R)-(+)-1(1-naphthyl)-ethylamine. Biotechnology Journal, 2023, 18: e2300120.
  9. Fangfang Fan#,  Chunyan Liu#, Jiaren Cao#, Changjiang Lyu, Shuai Qiu, Sheng Hu, Tingting Sun, Jiaqi Mei, Hongpeng Wang, Ye Li, Weirui Zhao, Lehe Mei, Jun Huang. Turning thermostability of Aspergillus terreus (R)-selective transaminase At-ATA by synthetic shuffling. Journal of Biotechnology, 2023, 364: 66-74.
  10. Lili Yao, Changjiang Lyu, Yuting Wang, Sheng Hu, Weirui Zhao, Hongwei Cao, Jun Huang, Lehe Mei. High-level production of 𝛾-aminobutyric acid via efficient co-expression of the key genes of glutamate decarboxylase system in Escherichia coli. Engineering Microbiology, 2023, 3: 100077.
  11. Keshun Hu, Ye Li, Feng Wang, Jianying Liu, Yuanyuan Li,  Qian Zhao, Xiaoye Zheng, Ningyu Zhu, Xiaoping Yu, Shaohua Fang, Jun Huang. A loop-mediated isothermal amplification-based microfluidic chip for triplex detection of shrimp pathogens. Journal of Fish Diseases, 2023,46(2),137-146.
  12. Fangfang Fan, Yongchao Zheng,  Yuzhuang Fu, Yuwei Zhang,  He Zheng,  Changjiang Lyu,  Linyu Chen,  Jun Huang,  Zexing Cao.  QM/MM and MM MD simulations on decontamination of the V-type nerveagent VX by phosphotriesterase: toward a comprehensive understanding of steroselectivity and activity, Physical Chemistry Chemical Physics, 2022, 24(18): 10933-10943
  13. Jia-Fei Lyu, Chang-Jiang Lyu, Jia-Ren Cao, Jia-Qi Mei, Sheng Hu, Wei-Rui Zhao, Tian-Yi Xu, Yu-Ting Wang, Dan-Li Wang, Jun Huang, Le-He Mei. High level production of γ-aminobutyric acid in engineered Escherichia coli by refactoring the glutamate decarboxylase, Process Biochemistry, 2022,118: 243-251
  14. Qian Zhao, Qing Li, Shuting Lin, Ruijun Hou, Jianying Liu, Yachen Tao, Ye Li, Yifeng Zhou, Zihong Ye, Xiaoping Yu, Jun Huang. De Novo transcriptome combined with physiological analyses revealed key genes for cadmium accumulation in Zhe-Maidong (Ophiopogon japonicus). Frontiers in Plant Science. 2022. DOI10.3389/fpls.2022.1078330
  15. Chun-Yan Liu#, Lupala Cecylia Severin#, Chang-Jiang Lyu#, Wan-Li Zhu, Hong-Peng Wang, Cheng-Jun Jiang, Le-He Mei, Hai-Guang Liu, Jun Huang. Improving thermostability of (R)-selective amine transaminase from Aspergillus terreus by evolutionary coupling saturation mutagenesis, Biochemical Engineering Journal, 2021, 167: 107926.
  16. Chang-Jiang Lyu, Jia-Yan Fei, Jiang-Ping Yan, Qi-Chun Xu, Jia-Qi Mei, Hui-Ying Yue, Lu-Lu Chen, Le-He Mei, Jun Huang, Shan-Jing Yao. Improvement of γ-aminobutyrate biosynthesis by genetically engineered Lactococcus lactis, Biochemical Engineering Journal, 2020, 157: 107525.
  17. Dong-Fang Xie, Jun-Xing Yang, Chang-JiangLv, Jia-Qi Mei, Hong-Peng Wang, Sheng Hu, Wei-Rui Zhao, Jia-Ren Cao, Jun-Liang Tu, Jun Huang, Le-He Mei. Construction of stabilized (R)-selective amine transaminase from Aspergillus terreus by consensus mutagenesis. Journal of Biotechnology, 2019, 293: 8-16.
  18. Changjiang Lyu, Weirui Zhao, Chunlong Peng, Sheng Hu, Hui Fang, Yujiao Hua, Shanjing Yao, Jun Huang, Lehe Mei. Exploring the contributions of two glutamate decarboxylase isozymes in Lactobacillus brevis to acid resistance and γ-aminobutyric acid production. Microbial Cell Factories, 2018,17(1): 180-194.
  19. Jun Huang, Bryan J. Jones, Romas J. Kazlauskas. Stabilization of an α/β-hydrolase by introducing proline residues: salicylic binding protein 2 from tobacco. Biochemistry, 2015, 54(28): 4330-4341.
  20. Jun Huang, Haihua Xie, Sheng Hu, Tian Xie, Jinyan Gong, Chengjun Jiang, Qing Ge, Yuanfeng Wu, Shiwang Liu, Yanli Cui, Jianwei Mao,  Lehe Mei. Preparation, characterization, and biochemical activities of N-(2-Carboxyethyl)chitosan from squid pens. Journal of Agricultural and Food Chemistry, 2015, 63: 2464−2471.
  21. Jun Huang, Wan-wen Chen, Sheng Hu, Jing-Yan Gong, Hong-Wu Lai, Peng Liu, Le-he Mei, Jian-wei Mao. Biochemical activities of 6-carboxy-β-chitin derived from squid pens. Carbohydrate Polymers, 2013, 91(1): 191-197.
  22. Jun Huang, Lehe Mei, Jiang Xia. Application of artificial neural network coupling particle swarm optimization algorithm to biocatalytic production of GABA. Biotechnology and Bioengineering, 2007, 96(5):924-931.
  23. 方卉, 吕常江, 花雨娇, 胡升, 赵伟睿, 方文姬, 宋奎, 黄俊, 梅乐和. 利用脯氨酸效应提高短乳杆菌谷氨酸脱羧酶的热稳定性. 生物工程学报, 2019, 35(4): 636-646.
  24. 谢东芳, 吕常江, 方卉, 杨卫康, 胡升, 赵伟睿, 黄俊*, 梅乐和*. 删除Loop 区域表面不稳定氨基酸提高(R)-ω-转氨酶热稳定性. 生物工程学报, 2017, 33(12): 1923-1933.

授权发明专利：

  1. 黄俊, 梅乐和, 吕常江, 朱婉丽, 王宏鹏, 胡升, 赵伟睿. 一种ω-转氨酶双突变体及其应用, 国家发明专利, ZL201910343331.8
  2. 黄俊, 梅乐和, 谢东芳, 吕常江, 朱婉丽, 胡升, 赵伟睿. 一种ω-转氨酶突变体及其应用, 国家发明专利, ZL201810619322.2
  3. 黄俊, 梅乐和, 朱婉丽, 吕常江, 胡升,  赵伟睿, 王宏鹏. 一种基于共进化网络的ω-转氨酶突变体以及制备方法和应用, 国家发明专利, ZL201811230792.6
  4. 黄俊, 谢东芳, 梅乐和, 王宏鹏, 胡升, 方卉, 赵伟睿, 吕常江. 一种引入二硫键的ω-转氨酶突变体及其应用, 国家发明专利, ZL201610456683.0
  5. 黄俊, 谢东芳, 方卉, 梅乐和, 王宏鹏, 胡升, 吕常江, 赵伟睿. ω-转氨酶突变体及其制备方法和应用, 国家发明专利, ZL201710308984.3
  6. 黄俊, 谢东芳, 楼坚, 蒋成君, 吴元锋, 龚金炎.ω-转氨酶突变体及其编码基因和制备方法, 国家发明专利, ZL201510901522.3.
  7. 黄俊, 梅乐和, 李佳男, 胡升, 谢湉, 谢东芳, 方卉. 一种羧基磁珠固定化谷氨酸脱羧酶及其制各方法和应用, 国家发明专利, ZL201610455225.5
  8. 黄俊, 叶慧, 谢湉, 林越呈, 张祥, 谢东芳, 方卉.一种N-羧乙基壳聚糖纳米磁珠及其制备方法和应用, 国家发明专利, ZL201610212609.4